10.22376/ijpbs.2019.10.1.p1-12 Volume 4 Issue 4 2013 (October - December) lessThan i greaterThan Orchis latifolia lessThan /i greaterThan belonging to the family Orchidaceae is extensively used in traditional Indian medicine against a wide spectrum of ailments including dysentery, diarrhoea, chronic fever, wounds, burns, fractures and general weakness. The present study relates to the bioactive extracts from lessThan i greaterThan O. latifolia lessThan /i greaterThan against multidrug resistant (MDR) bacteria and lessThan i greaterThan Candida albicans lessThan /i greaterThan . Methanolic extract of lessThan i greaterThan O. latifolia lessThan /i greaterThan was prepared and subsequently partitioned with various solvents. The extracts and fractions were evaluated for antimicrobial activities to identify the most active fractions. The bioactive fractions were studied by thin layer chromatography and direct bioautography. The lessThan i greaterThan n- lessThan /i greaterThan Hex fraction was identified as most active against MDR clinical isolates. The EtOAc fraction showed maximum activity against lessThan i greaterThan C. albicans lessThan /i greaterThan . Phytochemical analysis of the active fractions demonstrated the presence of flavanoids, steroids and tannins. The antimicrobial activity of lessThan i greaterThan O. latifolia lessThan /i greaterThan might be attributed due to the presence of alkaloids, flavanoids, steroids and tannins. The bioautography of these two active fractions exhibited the presence of few important chemical constituents which could serve as a promising lead against MDR target drug discovery. ANUPAMA SHARMA AVASTHI, SABARI GHOSAL AND SHARMISHTHA PURKAYASTHA Bioautography, Candida albicans, MDR clinical bacterial isolates, Orchis latifolia 638-646