10.22376/ijpbs.2019.10.1.p1-12 Volume 4 Issue 4 2013 (October - December) A study was carried out to isolate endophytes from lessThan i greaterThan Ocimum sanctum lessThan /i greaterThan (Tulsi) and check their anti-bacterial activitiy against lessThan i greaterThan Escherichia coli, Bacillus megaterium, Pseudomonas aeruginosa, Serratia marsences, Bacillus subtilis, Staphylococcus aureus, Micrococcus luteus, Salmonella typhi, Salmonella typhi A lessThan /i greaterThan and lessThan i greaterThan Salmonella typhi B lessThan /i greaterThan . Nine endophytes were isolated including five fungal and four bacterial endophytes and they were found to inhibit the test organisms significantly. Extracellular enzyme production was also assayed and 88.8% of total isolates were found positive for cellulase, 77.7% isolates were positive for pectinase, 55.5% isolates were positive for amylase and 77.7% were positive for tyrosinase. Ethyl acetate extract from fungal isolates were subjected to phytochemical analysis and they showed positive results for presence of alkaloid and flavonoid. These results suggest that endophytes from tulsi are potential to be used as antimicrobial agents and for production of enzymes as well as secondary metabolites. SUMAN SHEKHAWAT AND GAURAV SHAH Antibacterial activity, endophytes, enzymes, secondary metabolites, Ocimum sanctum. 600-607