10.22376/ijpbs.2019.10.1.p1-12 Volume 4 Issue 4 2013 (October - December) The crude latex extract of lessThan i greaterThan Asclepias curassavica lessThan /i greaterThan contains many proteins and exhibits strong proteolytic activity. The study was done to identify the clotting factor present in the latex of lessThan i greaterThan Asclepias curassavica lessThan /i greaterThan and to analyze the thrombin like activity of the clotting factor. In this study the plant latex from lessThan i greaterThan Asclepias curassavica lessThan /i greaterThan was collected and extracted the proteins. Proteolytic assays and inhibitor assays were carried out in the crude sample extract. The docking studies were carried out using Bioinformatics tools. The plasma clotting time was reduced from 143 seconds to 56 seconds in the presence of the crude latex extract. Reduction in coagulation time was observed with increase in the concentration of crude extract. lessThan i greaterThan In-silico lessThan /i greaterThan studies showed that the cysteine protease of lessThan i greaterThan Asclepias curassavica lessThan /i greaterThan interacts with the coagulation factors. The Factors VIII and XI there by leading to clot formation. Hence, from this research, it was proved that the compounds, Asclepain cI and cII, were readily involved in the clotting activity and were also found to reduce the time of coagulation. KARPAGAM .N, VISWANATHAN.S, PRABHU.S,SULOCHANA SOMASUNDARAM AND SIVANANDHAM.M Asclepias curassavica, Asclepain cII, Blood coagulation, Clotting Factor VIII and Factor XI, Cysteine protease, Thrombin. 542-552