10.22376/ijpbs.2019.10.1.p1-12 Volume 4 Issue 4 2013 (October - December) Infections caused by drug resistant Gram-negative bacteria are increasing day-by-day. An alternative mode of treatment needs to be considered to overcome the problem. With this aim in mind, the effect of Cefotaxime and aqueous extract of lessThan i greaterThan Terminalia chebula lessThan /i greaterThan was studied on lessThan i greaterThan Escherichia coli lessThan /i greaterThan by using Disc Diffusion Method and β-galactosidase assay. Disc Diffusion Method showed synergistic interaction between Cefotaxime and the aqueous extract of lessThan i greaterThan Terminalia chebula lessThan /i greaterThan against lessThan i greaterThan Escherichia coli lessThan /i greaterThan . The mode of action of the combination on lessThan i greaterThan Escherichia coli lessThan /i greaterThan was evaluated using β-galactosidase assay. An increase in the level of β-galactosidase enzyme was observed in the cell suspension treated with the combination as compared to the controls. These results suggest that the active components present in the aqueous extract of lessThan i greaterThan Terminalia chebula lessThan /i greaterThan should be an area of further lessThan i greaterThan in-vivo lessThan /i greaterThan research so as to find leads of compounds which can act in combination with antibiotics. ALPA RABADIA, S.D. KAMAT AND D.V. KAMAT Cefotaxime, Terminalia chebula, Disc Diffusion Method, β-galactosidase assay, synergistic interaction. 519-523