10.22376/ijpbs.2019.10.1.p1-12 Volume 4 Issue 4 2013 (October - December) The present study aimed at to evaluate the antimicrobial activity of methanol extracts of the endemic medicinal plant, lessThan i greaterThan Ceropegia pusilla lessThan /i greaterThan against five different bacterial strains namely, lessThan i greaterThan Shigella sonnei lessThan /i greaterThan MTCC 2957, lessThan i greaterThan Klebsiella pneumoniae lessThan /i greaterThan MTCC 109, lessThan i greaterThan Escherichia coli lessThan /i greaterThan MTCC 118, lessThan i greaterThan Staphylococcus aureus lessThan /i greaterThan ATCC 43300 and lessThan i greaterThan Bacillus lessThan /i greaterThan sp., and four different fungal strains namely, lessThan i greaterThan Aspergillus lessThan /i greaterThan sp., lessThan i greaterThan Mucor lessThan /i greaterThan sp., lessThan i greaterThan Penicillium lessThan /i greaterThan sp., and lessThan i greaterThan Candida albicans lessThan /i greaterThan ATCC 60192, by agar well diffusion method. The methanol extract from the lessThan i greaterThan in vitro lessThan /i greaterThan plant showed higher inhibitory effect towards the bacterium, lessThan i greaterThan Klebsiella lessThan /i greaterThan sp., moderate inhibitory effect against the fungal strains lessThan i greaterThan Penicillium lessThan /i greaterThan sp., and lessThan i greaterThan Candida albicans lessThan /i greaterThan . There is no inhibitory effect towards the other bacterial and fungal strains whereas the extract from lessThan i greaterThan in vivo lessThan /i greaterThan plant showed a higher inhibitory effect towards the bacterium lessThan i greaterThan Klebsiella lessThan /i greaterThan sp., and fungal strains lessThan i greaterThan Mucor lessThan /i greaterThan sp., lessThan i greaterThan Penicillium lessThan /i greaterThan sp., and lessThan i greaterThan Candida albicans lessThan /i greaterThan ., moderate inhibitory effect against lessThan i greaterThan Staphylococcus lessThan /i greaterThan sp., and no inhibitory effect against the other bacterial and fungal strains. On the basis of this experimental result, it can be concluded that methanol extract of lessThan i greaterThan in vitro lessThan /i greaterThan and lessThan i greaterThan in vivo lessThan /i greaterThan plants of lessThan i greaterThan Ceropegia pusilla lessThan /i greaterThan could be considered for further isolation and evaluation as therapeutic antimicrobial. K.KALIMUTHU R.PRABAKARAN AND C. BRINDHA Ceropegia pusilla, in vitro and in vivo plants, methanol extract, Antimicrobial activity. 187-191