10.22376/ijpbs.2019.10.1.p1-12 Volume 4 Issue 4 2013 (October - December) Urinary tract infection (UTI) is extremely common worldwide and numbers of patients are presenting to general practice and inpatient department. Gram negative bacteria are commonly involved in causing UTI. lessThan i greaterThan Escherichia coli lessThan /i greaterThan is found to be the most common causative agent of UTI. Prudent and rational use of antimicrobial is possible by forming local, national and global wide antibiogram. This study is done to find out the prevalence and antimicrobial susceptibility pattern of lessThan i greaterThan E.coli lessThan /i greaterThan causing urinary tract infection in tertiary care hospital, Bhopal. Total 1450 urine samples were collected and tested bacteriologically using standard procedures. Antimicrobial susceptibility testing was done by disk diffusion method described by Kirby-Bauer (1961). Culture positivity of urine samples was found to be 29 %.The most common pathogens were lessThan i greaterThan E.coli lessThan /i greaterThan (62%) followed by lessThan i greaterThan Klebsiella ( lessThan /i greaterThan lessThan i greaterThan 15.4 %), Enterobacter (6.9%), Pseudomonas (5.7%), Staphylococci aureus lessThan /i greaterThan lessThan i greaterThan (5.7%) lessThan /i greaterThan and others lessThan i greaterThan (4.3%) lessThan /i greaterThan . lessThan i greaterThan E.coli lessThan /i greaterThan was found to be most sensitive to imipenem followed by polymyxin-B, nitrofurantoin, gatifloxacin, colistin, doxycycline, amikacin and Gentamycin. ASATI RAKESH KUMAR AND SADAWARTE KALPANA UTI, E.coli, antibiotic susceptibility testing, antimicrobial resistance. 927-936