10.22376/ijpbs.2019.10.1.p1-12 Volume 4 Issue 4 2013 (October - December) Dye concentration of waste water can lead to a variety of problem. Biosorption has been proved to be a well established and cheap pollutant removal process among other purification techniques in this study our aim is to find the optimization of methylene blue by the spent biomass of lessThan i greaterThan Aspergillus terreus lessThan /i greaterThan using response surface methodology. The spent biomass was received as a free sample from Krebs Bio chemicals Limited, which was the discarded biomass from their lessThan i greaterThan Lovastatin lessThan /i greaterThan production plant. Box- Behnken design will be applied with 3 variables (dye concentration, adsorbent dosage and pH) in the chosen range so as to locate the optimal settings of the chosen variables. The spent biomass of lessThan i greaterThan Aspergilus lessThan /i greaterThan lessThan i greaterThan terreus lessThan /i greaterThan was found to be a potential low-cost biosorbent for the removal of methylene blue from water with a biosorption removal capacity of 94%. Box-Behnken design of RSM was proved to be a convenient tool for optimizing the biosorption process of dye removal, predicting a maximum methylene blue removal of 94% with the optimum process variables being found at 2.1 mg/100ml of initial methylene blue conc., 1.26 gm of adsorbent( lessThan i greaterThan A lessThan /i greaterThan . lessThan i greaterThan terreus lessThan /i greaterThan ) dosage/100 ml solution, and a pH value of 7.9. V. SRIDEVI Biosorption, Aspergillus terreus, RSM, agitation time, methylene blue concentration, adsorbent dosage, pH, temperature. 317-325