10.22376/ijpbs.2019.10.1.p1-12 Volume 4 Issue 3 2013 (July - September) In the present investigation, we have been able to establish a high regeneration protocol from leaves of lessThan i greaterThan Cichorium intybus lessThan /i greaterThan L., a medicinally important herb. Maximum number of shoots (8.58±0.31) with average length of 5.68 ± 0.20 cm was achieved on Murashige and Skoog (MS) medium fortified with 1.0 mgl lessThan sup greaterThan -1 lessThan /sup greaterThan Kinetin (KN) and 0.5 mgl lessThan sup greaterThan -1 lessThan /sup greaterThan Indole-3-acetic acid (IAA). Individual shoot implanted for rooting showed vigorous growth on MS medium supplemented with IAA (0.5 mgl lessThan sup greaterThan -1 lessThan /sup greaterThan ). Tissue culture raised plantlets were transferred for hardening in plastic pots containing soilrite and vermiculite (1:1). A total of 85.71% survival rate was recovered. Quantitative analysis of esculin content of lessThan i greaterThan in vitro lessThan /i greaterThan and field grown plantlets was carried out by High Pressure Liquid Chromatography (HPLC). Esculin production was phenological stage specific, in 6 weeks regenerants esculin was 4.78 ± 0.10 mg g lessThan sup greaterThan -1 lessThan /sup greaterThan dw, whereas 5.81 ± 0.12mg g lessThan sup greaterThan -1 lessThan /sup greaterThan dw in lessThan i greaterThan in vitro lessThan /i greaterThan flowering plants (10 weeks). ZAHID ALI, SHOWKAT. H. GANIE, ALKA NARULA, M. Z. ABDIN AND P. S. SRIVASTAVA Micropropagation, Cichorium intybus L, IAA, KN, Esculin 1333-1340