10.22376/ijpbs.2019.10.1.p1-12 Volume 4 Issue 3 2013 (July - September) Thermostable chitinase is produced by lessThan i greaterThan B. licheniformis lessThan /i greaterThan HSA3-1a from sulili hot springs in Pinrang, South Sulawesi which had been isolated and purified. Purification through ammonium sulphate precipitation step, dialysis, hydrophobic interaction chromatography with butyl sepharose FF matrix and gel filtration with sephadex G-75. The prepurification with ammonium sulphate precipitation results the highest chitinase activity on fraction 60-70% saturation. The results of hydrophobic interaction chromatography has two active fractions with highest activity on 40 lessThan sup greaterThan th lessThan /sup greaterThan and 43 lessThan sup greaterThan rd lessThan /sup greaterThan fractions, and the results of gel filtration chromatography to have an active fraction with the highest activity on 29 lessThan sup greaterThan th lessThan /sup greaterThan fraction. The results of purification were analyzed by SDS-PAGE showed a protein band at 97 kDa molecular weight estimates lessThan b greaterThan . lessThan /b greaterThan HASNAH NATSIR , ABD. RAUF PATONG, MAGGY T. SUHARTONO AND AHYAR AHMAD purification, characterization, chitinase, Bacillus licheniformis 1252-1259