10.22376/ijpbs.2019.10.1.p1-12 Volume 4 Issue 3 2013 (July - September) Though sixteen combinations of NAA + BAP, and NAA + KN, used in the study, induced callus from leaves of lessThan i greaterThan Coleus forskohlii lessThan /i greaterThan with a variable response, 0.5mg / l NAA + 1.5mg / l BAP was the most efficient (98.8 ± 1.1). Evaluation of hydro-alcoholic, methanolic and aqueous extracts of leaves and call us for antioxidant activity by DPPH and TBARS methods proved that hydro-alcoholic extracts were more efficient compared to others. Extracts of leaves and callus respectively exhibited lessThan i greaterThan IC lessThan sub greaterThan 50 lessThan /sub greaterThan lessThan /i greaterThan of 63.3µg/ml and 58.5µg/ml by DPPH; while only the callus extract exhibited 60µg/ml by TBARS. Proximity of callus IC lessThan sub greaterThan 50 lessThan /sub greaterThan of 58µg/ml to inhibition concentration indicates higher antioxidant activity compared to leaf extract. Quantification of polyphenols by HPLC exhibited three-fold increase in rosmarinic acid in callus (3.86mg/g), than leaves (1.25mg/g). Higher antioxidant activity of callus, due to rosmarinic acid, directs for further investigation into anti-inflammatory, analgesic and anti- depressant activity. K. SUNITHA, K. BHASKARA CHARY, CHETAN C. NIMGULKAR, B. DINESH KUMAR AND D. MANOHAR RAO Coleus forskohlii, Callus, HPLC, DPPH, TBARS, Rosmarinic acid 1139-1149