10.22376/ijpbs.2019.10.1.p1-12 Volume 4 Issue 3 2013 (July - September) Among the flowering plants, the members of cucurbitaceae are duly useful for human being that is agood source of vegetables and also a better resource for phytochemical compounds. The present study was aimed to induce the callus from lessThan i greaterThan in vitro lessThan /i greaterThan grown seedling explants. The effect of BAP and NAA have been investigated, the combination of BAP and NAA results in the higher callus induction. The callus was further analyzed for biochemical changes at 15 and 30 days of interval. The steep increase in phenol in 30 days callus compared to 15 days old callus, which could be one of the reason for browning of callus. J. JEROME JEYAKUMAR, M. KAMARAJ AND THIRUVENGADAM Cucumis anguria L, Cucumisanguria, Plant tissue culture 484-489