10.22376/ijpbs.2019.10.1.p1-12 Volume 4 Issue 3 2013 (July - September) lessThan i greaterThan Anopheles stephensi lessThan /i greaterThan mosquitoes were trapped from Karachi and its suburbs -Pakistan and examined for the detection of malarial parasites by nested polymerase chain reaction (PCR). lessThan i greaterThan Plasmodium lessThan /i greaterThan genus specific nested PCR amplification was applied on all samples. Only head and thorax of mosquitoes were used for pools preparation. Nested 1 product of lessThan i greaterThan Plasmodium spp lessThan /i greaterThan . positive samples were further processed for PCR amplification with species specific primers for lessThan i greaterThan P. falciparum lessThan /i greaterThan and lessThan i greaterThan P. vivax lessThan /i greaterThan . Total 610 pools of single mosquitoes were processed. Out of which 3.27% pools were found positive for lessThan i greaterThan P. falciparum lessThan /i greaterThan and 0.8% for lessThan i greaterThan P. vivax lessThan /i greaterThan and mixed infection was found in 2.13%. Nested PCR considered as a significantly high sensitive technique for the detection. SYEDA AZRA QAMAR AND IMTIAZ AHMAD Nested PCR; Anopheles stephensi; Plasmodium falciparum; Plasmodium vivax; Karachi- Pakistan. 222-228