10.22376/ijpbs.2019.10.1.p1-12 Volume 4 Issue 3 2013 (July - September) Pigeonpea ( lessThan i greaterThan Cajanus cajan lessThan /i greaterThan [L] Millsp.) is an important grain legume of the semi-arid tropics. It provides protein rich food. Seeds of pigeonpea were collected and surface sterilized and soaked overnight. This sterilized seeds were decoated and inoculated in MS media with various combination of cytokinin for cotyledonary node explants. Seeds inoculated were used for morphogenic response and various frequencies were observed using (0mg/l,1mg/l,2mg/l,3mg/l and 4mg/l) concentration of BAP, KIN and ZEA simultaneously. Highest percentage (88.35) of cotyledonary node explant formation was observed on MS media + 2.0 mg/l BAP, 3% sucrose, 0.7% (w/v) agar. JHARNA SRIVASTAVA, PRAMOD K. RAGHAV AND ANIRUDHA RISHI Cajanus cajan, cotyledonary node explant, MS media, BAP, Kinetin, Zeatin. 55-63