10.22376/ijpbs.2019.10.1.p1-12 Volume 4 Issue 3 2013 (July - September) To establish a novel methodology for the evaluation of prophylactic property of drugs/ plant products against experimentally induced diabetes in animal models. Test drug is administered by oral/ i.v. route to animals every day for 90 days. On 91st day, diabetes is induced by chemical method. Test drug administration is continued for another 4 days i.e. from 91 lessThan sup greaterThan st lessThan /sup greaterThan to 94 lessThan sup greaterThan th lessThan /sup greaterThan day. CBG, lipid profile, body weight are measured once in 15 days till 90 days and everyday for next 10 days by any standard method. After 3 lessThan sup greaterThan rd lessThan /sup greaterThan day of diabetes induction, test rats are not given any drugs, but regular feed is continued upto 10 lessThan sup greaterThan th lessThan /sup greaterThan day. CBG is recorded on 2 lessThan sup greaterThan nd lessThan /sup greaterThan /3 lessThan sup greaterThan rd lessThan /sup greaterThan day after diabetes induction and analysed as:If average CBG/ lipid levels in the test group is :- i)Same or more compared to the diabetic control group: infers that there is no prophylactic activity of the evaluating product. ii) Less than the diabetic control group: infers that the test evaluating product has antihyperglycemic activity indicating the possibility of prophylactic property. ii) Less than the non-diabetic control group at any time of the experiment (1st to 94 lessThan sup greaterThan th lessThan /sup greaterThan day): infers that the evaluating product has a positive tendency of hypoglycemia with possibility of treatment property. This novel methodology of investigating a drug for its prophylactic property against chemically induced diabetes in animal models may be useful to evaluate a product with future perspective targeting preventive approach in prediabetics or high risk individuals of type 2 diabetes and normal individuals. This novel approach may be useful to screen drugs / plant products / neutraceuticals likely to postpone onset of diabetes or development of its complications. SURESHA R.N, SUSHMA V NAIDU, SATISH A M, KALABHARATHI H L AND PUSHPA V H prophylactic, animal diabetic model, antihyperglycemic, chemical method. 1012-1016