10.22376/ijpbs.2019.10.1.p1-12 Volume 4 Issue 3 2013 (July - September) Grape ( lessThan i greaterThan Vitis vinifera lessThan /i greaterThan ) seed extract found to be exhibits wide range of beneficial effects insights to screen the potential compounds which includes polyphenolic compounds both flavonoids and non-flavonoids that show antioxidant effects. In this present study phytochemical screening of alkaloids, flavonoids, glycosides, polyphenol and flavonoids, tannins, and sterols were presented in the ethanolic grape seed extract. The lessThan i greaterThan In vitro lessThan /i greaterThan antioxidant activity was estimated by IC lessThan sub greaterThan 50 lessThan /sub greaterThan value and the values are65.44 µg/ml for DPPH radical scavenging assay and inhibition of hydroxyl radical, superoxide anions, lessThan b greaterThan hydrogen peroxide anions and lipid peroxide inhibition assay with IC lessThan sub greaterThan 50 lessThan /sub greaterThan values of 77.33, 92.43, 88.15 and 83.54 lessThan /b greaterThan µg/ml, respectively. lessThan b greaterThan The results obtained from this study clearly indicate that lessThan /b greaterThan ethanolic grape ( lessThan i greaterThan Vitis vinifera lessThan /i greaterThan ) seed extract lessThan b greaterThan possesses antioxidant activity. lessThan /b greaterThan S.SWAPNA REKHA AND M.BHASKAR. Grape (Vitis vinifera) seed extract, phyto-chemical screening, polyphenolic compounds, In vitroantioxidant. 609-617