10.22376/ijpbs.2019.10.1.p1-12 Volume 4 Issue 3 2013 (July - September) A simple, precise, rapid and accurate reverse phase HPLC method has been developed for the determination of Paclitaxel in bulk and its pharmaceutical dosage form. An enable C18G, 250mmX4.6mm i.d, 5µm particle size column was used with photo diode array UV-Visible detector. The mobile phase consisting of Phosphate Buffer p lessThan sup greaterThan H lessThan /sup greaterThan 5.0 and Acetonitrile in the ratio of 90:10V/V was used. The flow rate was 1ml/min and the effluent was monitored at 282nm. The retention time of the drug was 3.610 minutes. The method was linear over the concentration range of 25-125µg/ml. the method precision for the determination of assay was below 2% RSD. The percentage recovery of paclitaxel was 99.41 – 99.83%. The validation of method was carried out utilizing ICH guidelines. SREERAMAMURTHY PYLA., K.SRINIVAS., JOGENDRAKUMAR YVV. AND JAGADEESH PANDA. Paclitaxel, RP- HPLC, Development, Validation. 534-540