10.22376/ijpbs.2019.10.1.p1-12 Volume 4 Issue 3 2013 (July - September) A novel, simple, accurate, sensitive, reproducible, economical spectroscopic method has been developed and validated for the determination of Nebivolol hydrochloride and S-amlodipine besylate in combined dosage form. The method obeys Beer's Law in concentration range of 15-35 µg/ml for Nebivolol and 7.5-17.5 µg/ml for S-amlodipine. The method was validated for linearity, range, accuracy, precision and specificity as per ICH guidelines. Zero crossing point for Nebivolol hydrochloride and S-amlodipine besylate was 243 nm and 291 nm respectively in methanol. The developed method as successfully used for the quantitative analysis of commercially available dosage form (Nebicard SM). DR. ATUL PATEL Nebivolol hydrochloride, S-amlodipine besylate, Derivative Spectroscopy, Zero crossing point, combined dosage form. 379-391