10.22376/ijpbs.2019.10.1.p1-12 Volume 4 Issue 2 2013 (April - June) Constant surveillance of microbial pathogens is essential for combating infectious diseases which account for at least a quarter of all illnesses. This prospective study was designed to provide information on the incidence of Gram-positive bacterial infections in King Abdulaziz University Hospital (KAUH), Jeddah, Saudi Arabia. A total of 98 Gram-positive isolates were obtained and identified. Their antibiotic susceptibilities were documented against several antibiotics. All methicillin-resistant lessThan i greaterThan Staphylococcus aureus lessThan /i greaterThan (MRSA) were examined for the existence of the lessThan i greaterThan Staphylococcus aureus lessThan /i greaterThan specific gene and lessThan i greaterThan mecA lessThan /i greaterThan gene by polymerase chain reaction. The most common individual isolates were staphylococci (62.2%) followed by enterococci (30%), then streptococci (0.05%). Twenty nine percent of the isolates were recovered from wounds and the highest incidence was in intensive care units (30.61%). Twenty percent of MRSA isolates were multidrug resistance. Glycopeptide resistances were detected in two isolates of lessThan i greaterThan Enterococcus faecium. lessThan /i greaterThan All strains were sensitive to linezolid and tigycycline. Molecular technique found that all the isolates of MRSA had lessThan i greaterThan S. aureus lessThan /i greaterThan specific gene and lessThan i greaterThan mecA lessThan /i greaterThan gene. In conclusion, the incidence of resistance in Gram-positive cocci causing infections in KAUH is an increasing problem and molecular techniques using lessThan i greaterThan mecA lessThan /i greaterThan gene can be used to detect MRSA. N. R. HELMI, R. M. ZAMAN AND M. M. ALY Antimicrobial resistance, MRSA, molecular typing, S.aureus, mecA gene, Gram positive. 1231-1245