10.22376/ijpbs.2019.10.1.p1-12 Volume 4 Issue 2 2013 (April - June) There are several water-borne diseases which are highly pathogenic for humans. Amongst all, genus lessThan i greaterThan Vibrio lessThan /i greaterThan plays a major role. In this case study of "RAPID IDENTIFICATION OF HUMAN PATHOGENIC VIBRIO SPECIES USING MULTIPLEX PCR" We have chosen salt water bodies such as sea and estuaries for sampling. To facilitate the identification of human-pathogenic species, we have used multiplex PCR to amplify gene regions in five species ( lessThan i greaterThan V. cholerae lessThan /i greaterThan ,  lessThan i greaterThan V. parahaemolyticus lessThan /i greaterThan ,  lessThan i greaterThan V. vulnificus lessThan /i greaterThan , lessThan i greaterThan V. mimicus and V. alginolyticus lessThan /i greaterThan ). The assay was tested on a sample of 82  lessThan i greaterThan Vibrio lessThan /i greaterThan  isolates for identification of five common human pathogenic vibrio species. Multiplex PCR was performed targeting the tox gene present in the five most common pathogenic vibrio species. After analysis of all the results we have found that salt water is having a higher concentration of vibrio species than freshwater & lessThan i greaterThan V.vulnificus lessThan /i greaterThan was most abundant among all. REENA KUMARI, AMIT RANJAN PRASAD, SHASHANK GUPTA, SHIPRA MOHAN TRIPATHI, SHWETA SINGH, PRASHANT VIKRAM SINGH, MADHULIKA SINGH, SHIVANI SINGH,TUSHAR JOSHI, SUBHENDRA AND SUJEET KUMAR SINGH Multiplex PCR, Halophilic, tox gene, Human pathogenic. 1117-1124