10.22376/ijpbs.2019.10.1.p1-12 Volume 4 Issue 2 2013 (April - June) The aim of this study was to investigate antioxidant, antibacterial, cytotoxic and apoptotic activities of lessThan i greaterThan Annona lessThan /i greaterThan lessThan i greaterThan squamosa lessThan /i greaterThan L seed petroleum ether extract (ASPE). Antioxidant activity was assessed by DPPH scavenging activity, antibacterial activity was tested using broth dilution technique and antiproliferative activity was evaluated by MTT assay. The apoptosis inducing capacity was detected by Acridine Orange –Ethidium Bromide Staining, Hoechst Staining and Annexin V expression was assessed by flowcytometry. The extract showed antimicrobial activity and free radical scavenging activity, RSA lessThan sub greaterThan 50 lessThan /sub greaterThan of ASPE was 70.5 µg/ml. The active fraction showed IC lessThan sub greaterThan 50 lessThan /sub greaterThan value 33.8 µg/ml of KB cells. Acridine Orange Ethidium Bromide and Hoechst staining exhibited apoptotic features and Flowcytometry revealed increased levels of Annexin-V expression after treatment with the extract. These results suggest that the active fraction isolated from lessThan i greaterThan Annona lessThan /i greaterThan lessThan i greaterThan squamosa lessThan /i greaterThan seed extracts have strong antibacterial, antioxidant and antitumor activities. BIBA, V. S; JEBA MALAR, P.W AND REMANI, P Annona squamosa, antioxidant, antibacterial, cytotoxic, apoptotic 899-907