10.22376/ijpbs.2019.10.1.p1-12 Volume 4 Issue 2 2013 (April - June) This experimental research is aimed to meet the industrial demand of lipase enzyme using various supplements along with the optimal physical conditions for enhancing the production. lessThan i greaterThan Bacillus licheniforms lessThan /i greaterThan KDP was used in this study which has been isolated from the oil mill soil. In the present investigation, selenium is used as a media supplement which has not been previously reported as supplement and thus reported by us for the first time. The maximum lipase production (16.23U/ml) was found to be at 60μm/l. Another metal ion, nickel when separately used as supplement to the medium, the maximum production (17.29U/mL) was found to be 80μm/L. The new isolate produced their maximum extracellular lipase at 7.5 (18.69 U/mL) and 45°C (17.36U/mL). The maximum lipase yield 12.43U/mL and15.38U/mL was found to be in the medium containing 0.1mg/l urea and 1mg/l ammonium nitrate respectively. The correlation analyses of all the experiments proved that the physicochemical parameters and the lipase production are interdependent. K . SUJATHA AND K. DHANDAYUTHAPANI Ammonium nitrate, Bacillus licheniforms KDP, Lipase, Nickel, Selenium, Temperature Urea, pH, 645-652