10.22376/ijpbs.2019.10.1.p1-12 Volume 4 Issue 2 2013 (April - June) Multilocus enzyme electrophoresis data from 14 orthologous loci (25 alleles) were used to infer the genetic variation between the two Tephritidae species, lessThan i greaterThan Bactrocera (Dacus lessThan /i greaterThan ) lessThan i greaterThan cucurbitae lessThan /i greaterThan and lessThan i greaterThan B. lessThan /i greaterThan ( lessThan i greaterThan D. lessThan /i greaterThan ) lessThan i greaterThan dorsalis lessThan /i greaterThan , which are common pests of vegetables and fruits in India. Out of fourteen loci analyzed, seven loci were polymorphic in lessThan i greaterThan B. cucurbitae lessThan /i greaterThan and nine loci were polymorphic in lessThan i greaterThan B. dorsalis. lessThan /i greaterThan The amount of observed (0.18) and expected heterozygosity (0.26) in lessThan i greaterThan B. cucurbitae lessThan /i greaterThan were lower than the observed (0.33) and expected heterozygosity (0.40), in lessThan i greaterThan B. dorsalis lessThan /i greaterThan . The estimates of genetic variation in terms of observed and expected heterozygosity were higher as compared to the general low level of variability found in tephritid fruit flies. Nei's genetic identity value (0.833) reveals a very close genetic similarity between the two species. RASHMI SINGH AND AKANKSHA SINGH Allozymes, Dacus, molecular markers, heterozygosity, Hardy-Weinberg equilibrium, genetic identity 617-623