10.22376/ijpbs.2019.10.1.p1-12 Volume 4 Issue 2 2013 (April - June) Chlorpyrifos is an organophosphorus pesticide and widely used in the agricultural field to control beetles, whiteflies, bugs, and flies like insects. This investigation describes the biodegradation of chlorpyrifos by lessThan i greaterThan Pseudomonas desmolyticum lessThan /i greaterThan NCIM 2112. The biodegradation is influenced by other carbon and nitrogen sources and indicates that glucose and maltose are effective at 0.5% concentration and NaNO lessThan sub greaterThan 3 lessThan /sub greaterThan and yeast extract at 0.05%. The pH and temperature optima for degradation was found to be 7.0 and at 30 lessThan sup greaterThan 0 lessThan /sup greaterThan C respectively. ­­­­­­­­­­­­ lessThan i greaterThan Pseudomonas desmolyticum lessThan /i greaterThan NCIM 2112 degrades chlorpyrifos into non toxic metabolites like 2-pyridinol and thiophosphate. KEDAR B ROKADE AND GAJANAN V MALI Pesticide, Chlorpyrifos, Pseudomonas, Biodegradation. 609-616