10.22376/ijpbs.2019.10.1.p1-12 Volume 4 Issue 2 2013 (April - June) lessThan i greaterThan Aerides maculosum lessThan /i greaterThan Lindl., is an endemic orchid species of Western Ghats, India. In this study, an efficient and reproducible protocol was developed for lessThan i greaterThan in vitro lessThan /i greaterThan seed germination. The seeds were cultured on MSBM supplemented with variable concentrations of coconut water (CW) and 6-BAP, casein hydrolysate (CH) and banana pulp. 87%-91% seeds were found viable by differential staining. MSBM supplemented with 10% CW, 2mg/l 6-BAP and 500mg/l CH exhibited best (95.6%) germination rate. Effect of MSBM in combinations of growth regulators: 1-NAA, IAA 6-BAP, kinetin on protocorm were studied. MSBM fortified with 6-BAP (2mg/l) and 1-NAA (1mg/l), resulted in 3.78±1.81 shoots per plantlet and 2.62±1.68 roots per plantlet. The healthy axenic plants with well developed roots were transferred to peat: perlite: vermiculite (1:1:1v/v) medium for 90 days, followed by the medium brick chips: charcoal: mosses (1:1:1v/v) for six months. The acclimatized plants were reintroduced to the nature with 77% survival frequency. SRIVASTAVA DEEPTI GAYATRI M.C. AND SARANGI SITIKANTHA Aerides maculosum Lindl.; Murashige and Skoog basal medium (MSBM); 6-benzyl aminopurine (6-BAP); casein hydrolysate (CH); protocorm. 478-486