10.22376/ijpbs.2019.10.1.p1-12 Volume 4 Issue 2 2013 (April - June) Three alkaline protease producing gram positive, spore forming lessThan i greaterThan Streptomyces lessThan /i greaterThan (J1, J2 and J3) were isolated from alkaline soil (pH range: 9-10) samples collected from Central Soil Salinity Research Institute (C.S.S.R.I), Lucknow. On the basis of halo zone, morphology, texture and microscopic characteristics they were identified as lessThan i greaterThan Streptomyces lessThan /i greaterThan . Further characterization both genetically and phenotyphically were done in order to establish their phylogeny. The results of the 16S rDNA sequences were analysed using BLASTn. On the basis of the homology results the isolates ((J1, J2 and J3) were identified as lessThan i greaterThan S. macrosporeus, S. bacillaris, lessThan /i greaterThan and lessThan i greaterThan S. rubrolavendulae, lessThan /i greaterThan respectively lessThan i greaterThan . lessThan /i greaterThan Optimization studies under different carbon and nitrogen sources at pH 10 under 72 h of incubation revealed maximum protease production by lessThan i greaterThan S. rubrolavendulae lessThan /i greaterThan (12.50 U/ml) and lessThan i greaterThan S. bacillaris lessThan /i greaterThan (8.82U/ml) and the minimum by lessThan i greaterThan S. macrosporeus lessThan /i greaterThan (0.95 U/ml). The crude alkaline protease thus revealed maximum activity at 37 lessThan sup greaterThan 0 lessThan /sup greaterThan C at pH 10.  JYOTI VERMA, SHIKHA, DINESH RAJ MODI AND SANGEETA SAXENA Alkaline protease, Streptomyces, 16S rDNA 214-224