International Journal of Pharma and Bio Sciences
ijpbs.net
editorijpbs@rediffmail.com (or) editorofijpbs@yahoo.com (or) prasmol@rediffmail.com
10.22376/ijpbs.2019.10.1.p1-12
Volume 4 Issue 2
2013 (April - June)
CHARACTERIZATION OF THE Α-AMYLASE INHIBITOR FROM THE SEEDS OF MACROTYLOMA UNIFLORUM AND VIGNA UNGUICULATA
α-amylase inhibitors were purified and characterized from the seeds of lessThan i greaterThan Macrotyloma uniflorum lessThan /i greaterThan (MUAI) and lessThan i greaterThan Vigna unguiculata lessThan /i greaterThan (VUAI). MUAI was purified using CM-cellulose column while VUAI was purified using Poros HS-50 column followed by gel filtration on Sephadex G-75 column. The molecular weights of MUAI and VUAI as determined by gel filtration were 26.91 kD and 21.89 kD respectively. Both MUAI and VUAI were found to be glycoproteins. The secondary structure of both MUAI and VUAI was determined using circular dichroism studies at different pH and different temperatures. MUAI and VUAI were identified using MALDI-TOF/TOF and LC MS lessThan sup greaterThan E lessThan /sup greaterThan analysis respectively.
LAXMI H. GUPTA, ASHOK CHOUGALE, MAHESH KULKARNI AND SUSHMA G. SABHARWAL
α-amylase inhibitor, Circular dichroism, glycoprotein, MALDI-TOF/TOF, TLC
127-137