10.22376/ijpbs.2019.10.1.p1-12 Volume 4 Issue 2 2013 (April - June) The fungal populations in the alkaline soil samples collected from Eastern Ghats, Kolli hills region was studied by using potato dextrose agar medium. The samples possessed an average of 15X10 lessThan sup greaterThan 4 lessThan /sup greaterThan colony forming unit per gram of soil. Among this population 23 strains of fungi belong to five genus such as lessThan i greaterThan Trichoderma, Aspergillus, Penicillium, Rhizopus lessThan /i greaterThan and lessThan i greaterThan Mucor lessThan /i greaterThan were isolated. Protease producing fungal species was screened by Casein and Skimmed milk agar. Among the isolates three strains of lessThan i greaterThan Aspergillus lessThan /i greaterThan spp., one lessThan i greaterThan Mucor lessThan /i greaterThan sp lessThan i greaterThan . lessThan /i greaterThan and one strain of lessThan i greaterThan Curvularia lessThan /i greaterThan sp lessThan i greaterThan . lessThan /i greaterThan showed greater proteolytic activity in both of the medium. An isolate of lessThan i greaterThan Aspergillus lessThan /i greaterThan strain KH 17 showed maximum zone of proteolysis in screening media and greater enzyme activity in submerged fermentation 215U/ml in complex media containing casein. The effect of physiochemical parameters studied includes different carbon, nitrogen sources, pH and temperature. Among the parameters tested 1 % starch, 3% soya bean meal, pH 9 and temperature of 25 to 30 lessThan sup greaterThan 0 lessThan /sup greaterThan C showed greater induction in enzyme production. The crude enzyme of cell free extract was concentrated by ammonium sulphate and dialysis. The precipitate showed 4.1 fold greater activities and dialyzed precipitate showed 6.7 fold greater specific activity per milligram protein than the crude. The fibrinolytic activity of the partially purified enzyme was determined, it showed prompt fibrinolytic activity. The area of clearance by alkaline protease was observed to be 64.5 ± 2.4 mm lessThan sup greaterThan 2 lessThan /sup greaterThan . From this study it is found that the alkaline protease isolated from lessThan i greaterThan Aspergillus lessThan /i greaterThan strain KH 17 has a great scope of medicinal, diagnostic and industrial applications. P. PALANIVEL, L. ASHOKKUMAR AND R. BALAGURUNATHAN Alkaline protease, Aspergillus spp., Partial purification, Characterization, Fibrinolytic action. 101-110