10.22376/ijpbs.2019.10.1.p1-12 Volume 4 Issue 2 2013 (April - June) Phospholipase A2 isolated from venoms is a potential new antibacterial source. Antibacterial activity tests of phospholipase A lessThan sub greaterThan 2 lessThan /sub greaterThan were performed against lessThan i greaterThan Staphylococcus aureus lessThan /i greaterThan and lessThan i greaterThan Bacillus subtilis lessThan /i greaterThan . Phospholipase A lessThan sub greaterThan 2 lessThan /sub greaterThan which was isolated from spines venom of lessThan i greaterThan Acanthaster planci lessThan /i greaterThan by using fractionated 20% saturated ammonium sulfate precipitation and its antibacterial assay was done by using disc diffusion method whereas 40 μl isolate at a concentration of 6.29 μg/μl was applied onto 7 mm paper disc. The assay exhibited inhibition zone around the phospholipase A lessThan sub greaterThan 2 lessThan /sub greaterThan disc against lessThan i greaterThan Staphylococcus aureus lessThan /i greaterThan while no inhibition zone against lessThan i greaterThan Bacillus subtilis lessThan /i greaterThan . As a result, phospholipase A lessThan sub greaterThan 2 lessThan /sub greaterThan posses an antibacterial activity against lessThan i greaterThan Staphylococcus aureus lessThan /i greaterThan . FERA IBRAHIM, NUNUK WIDHYASTUTI, IMELDA KRISANTA ENDA SAVITRI, MUHAMAD SAHLAN AND ANONDHO WIJANARKO Phospholipase A2, venom, Acanthaster planci, antibacterial activity 01-05