10.22376/ijpbs.2019.10.1.p1-12 Volume 4 Issue 2 2013 (April - June) Isolation and purification of bioactive compounds from naturally occurring substance will play a central role in the development and modernization of phytomedicine.The study was carried out to the isolate and simultaneous identification of gallic acid from the Ethanolic Extract of lessThan i greaterThan Polyalthia Longifolia lessThan /i greaterThan (Sonn.) Thawaites leaves (EEPL). The simple, rapid and cost effective Preparative Thin Layer Chromatography (PTLC) was used for the identification and isolation of gallic acid. R lessThan sub greaterThan f lessThan /sub greaterThan value of PTLC isolate and reference standard gallic acid was found to be 0.47 and 0.48 respectively. Reverse Phase High Performance Liquid Chromatography (RP-HPLC) fingerprint of PTLC isolate of EEPL also revealed that the presence of gallic acid. SAMPATH M Polyalthia longifolia, Phytomedicine, Phenolic acid, PTLC isolate, Antioxidant. 966-972