10.22376/ijpbs.2019.10.1.p1-12 Volume 4 Issue 2 2013 (April - June) The aim of the present investigation deals with biological evaluation lessThan i greaterThan Peucedanum Beluchistanicum leaves. lessThan /i greaterThan For this purpose different biological assay of methanolic extract (Crude) and its fractions that are chloroform fraction, lessThan i greaterThan n lessThan /i greaterThan -hexane fraction, Ethyl acetate fraction, lessThan i greaterThan n- lessThan /i greaterThan butanol fraction and aqueous fraction were carried out. The results from the agar diffusion method indicated that Crude showed maximum antibacterial activity against lessThan i greaterThan Staphylococcus aureus lessThan /i greaterThan with the inhibition zone (28.3±0.04mm). Whereas Ethyl acetate fraction also showed strong activity against lessThan i greaterThan Staphylococcus aureus lessThan /i greaterThan (25.2±0.9mm). On the other hand, Crude showed maximum activity against lessThan i greaterThan Candida albicans lessThan /i greaterThan and lessThan i greaterThan Candida glaberata lessThan /i greaterThan with % inhibition of (73±0.4%) and (68±0.2 %) respectively and Chloroform fraction showed good activity against lessThan i greaterThan Candida albicans lessThan /i greaterThan with % inhibition of (62±0.12%). Furthermore, Crude showed maximum insecticidal % mortality against lessThan i greaterThan Tribolium castaneum lessThan /i greaterThan with (86%) mortality whereas (80%) mortality against lessThan i greaterThan Rhyzopertha dominica. lessThan /i greaterThan and also Chloroform fraction also showed maximum activity against lessThan i greaterThan Tribolium castaneum lessThan /i greaterThan with (70%) mortality. Moreover Chloroform fraction showed significant Cytotoxic activity with ED lessThan sub greaterThan 50 lessThan /sub greaterThan value 0.85 µg//ml and Crude showed Cytotoxic activity with ED lessThan sub greaterThan 50 lessThan /sub greaterThan value 0.98 µg//ml. Furthermore, the phytochemical analysis of Crude and its fractions showed the presence of Alkaloids, Flavonoids, Phenols, Saponins and Diterpenes. NIZAM BALOCH, ABDUL MANAN KAKAR, SAJID NABI,MASOOM YASINZAI AND DR. YASSER M.S.A. AL-KAHRAMAN Antimicrobial, Insecticidal, Cytotoxic Activity, Phytochemical Analysis, Peucedanum Beluchistanicum Leaves 898-905