10.22376/ijpbs.2019.10.1.p1-12 Volume 4 Issue 2 2013 (April - June) The mycobacterial cells were treated with 0.96 mg/ml of Ethambutol (EMB) and 0.64 mg/ml of Rifampicin (RIF) in Luria Bertani (LB) broth. The native and treated cells were serially diluted (10 lessThan sup greaterThan -1 lessThan /sup greaterThan to 10 lessThan sup greaterThan -30 lessThan /sup greaterThan ), from the 10 lessThan sup greaterThan -25 lessThan /sup greaterThan dilution native cells and antibiotics treated cells were imaged using Atomic Force Microscopy (AFM). The normal surface roughness (R lessThan sub greaterThan rms lessThan /sub greaterThan ) of the native cells was 0.353 nm, whereas the surface roughness for EMB treated cells was 3.14 nm. The RIF treated cells pores (width size: 19.86 nm) were created on the surface of the cell membrane. This paper provides a new finding on EMB was alone creates the roughness on the cell wall surface of lessThan i greaterThan Mycobacterium smegmatis lessThan /i greaterThan . Interestingly we got the better image of after treatment of RIF was creates pores on the cell wall surface of lessThan i greaterThan M. smegmatis. lessThan /i greaterThan P. SANKAR GANESH , K. KANIVALAN, K. RAJENDRAN,D.KUMAR AND S. KUMARAN Mycobacterium smegmatis, Ethambutol, Rifampicin, Roughness, Pores 255-262