10.22376/ijpbs.2019.10.1.p1-12 Volume 4 Issue 2 2013 (April - June) To develop a reliable, ecofriendly procedure for bioreduction of silver nanoparticles by lessThan i greaterThan Aspergillus flavus. lessThan /i greaterThan Methods: Characterization was done by using UV-VIS spectrophotometry, Infrared spectroscopy (FT-IR) and X-ray diffraction. Results: IR spectroscopy showed the potential biomolecule responsible for the reduction of silver nanoparticles and stability of the bioreduced silver nanoparticles. X-ray diffraction analysis showed that the particles were crystalline in nature with face centered cubic geometry. Conclusion: lessThan i greaterThan A.flavus lessThan /i greaterThan was found to be a good producer of silver nanoparticles by releasing the extracellular enzyme nitrate reductase. Silver nanoparticles are known to have inhibitory and bactericidal effects against MDR strains of lessThan i greaterThan Pseudomonas aeruginosa, Escherichia coli lessThan /i greaterThan and lessThan i greaterThan Klebsiella pneumonia lessThan /i greaterThan . Resistance of bacterial infections has emerged in recent years and is a major health problem. Here, we report the extra cellular biosynthesis of silver nanoparticles using the fungus, lessThan i greaterThan Aspergillus flavus lessThan /i greaterThan and its antibacterial activities against pathogenic MDR strains. SHIVARAJ NINGANAGOUDA,VANDANA RATHOD,JYOTI.H,DATTU SINGH,PREMA.K AND MANZOOR-UL-HAQ Aspergillus flavus, FT-IR, MDR strains, Silver nanoparticles, XRD. 222-229