10.22376/ijpbs.2019.10.1.p1-12 Volume 4 Issue 2 2013 (April - June) A HPTLC method was established for assay of 6-Gingerol and determination from herbal oral thin film (OTF).The chromatographic condition were: Toluene: Ethyl acetate: methanol: Formic Acid (5: 4:1:1v/v/v/v) as mobile phase; wavelength of the detector was 520nm; drying temperature after development was 60°C. The calibration curve was linear in the range of 100 to 600mcg/ml. With correlation coefficient of 0.999; the average recovery was found to be 100.24%, 100.45% and 100.49% at the concentration levels of 80, 100 and 120% respectively. Detection and quantification limit was fond to 80 and 120ng. The method was found to be simple, reliable, accurate and precise in accordance with ICH guidelines. SAFEENA SHEIKH, SUHAIL ASGHAR AND SHOWKAT AHMAD 6-Gingerol, Ginger, OTF, method development, validation, densitometry, Quantification and chromatography. 93-98