10.22376/ijpbs.2019.10.1.p1-12 Volume 4 Issue 1 2013 (January - March) The purpose of the study was to prepare and optimize a sustained release formulation of isoniazid microspheres and to study the effect of lessThan i greaterThan Piper nigrum lessThan /i greaterThan (Black Pepper) on its lessThan i greaterThan in-vitro lessThan /i greaterThan release. Microspheres containing isoniazid were prepared using double emulsification and complex coacervation methods. The critical formulation variables were concentration of polymer, drug - polymer ratio, cross-linking agent concentration and cross-linking time. The time to release 85% of the contents of the microspheres ( lessThan i greaterThan t lessThan /i greaterThan lessThan sub greaterThan 85 lessThan /sub greaterThan ) was used as the measure for the release time of the drug. The microspheres were optimized on the basis of their particle size, percentage yield, entrapment efficiency, bioadhesion study and lessThan i greaterThan in vitro lessThan /i greaterThan drug release. The lessThan i greaterThan in-vitro lessThan /i greaterThan release of the optimized batches of isoniazid microspheres was enhanced to the extent of 100-107%, by coadministration of 10 or 15 mg of bioenhancer. PRAHSANT L. PINGALE AND RAVINDRA R.P. Tuberculosis, Bioenhancer, Piperine, Isoniazid, Microsphere 1027-1036