10.22376/ijpbs.2019.10.1.p1-12 Volume 4 Issue 1 2013 (January - March) In the present study mitochondrial DNA sequences i.e. Cytochrome oxidase I (CO I) and Control region (CR) are characterized in order to study the genetic variability and phylogenetic relationships among flies belonging to the family Calliphoridae. Since, these flies are of medical and veterinary importance, therefore, it is imperative to use molecular markers to resolve the evolutionary mechanism responsible for specific genetic variability. The data reveals that there is very little genetic difference among these species. The phylogenetic tree derived by distance and character-state approach shows similar topologies. BAJPAI N., MALVIYA S. AND TEWARI R. R. Calliphoridae, Control region, Cytochrome oxidase , Phylogeny. 973-977