10.22376/ijpbs.2019.10.1.p1-12 Volume 4 Issue 1 2013 (January - March) Experiments have been carried out to investigate the DNA base specificity of light-induced banding (LIB) produced by photo-oxidation of chromosomes followed by Quinacrine staining to detect denatured DNA. In the present study mitotic preparations from lessThan i greaterThan Sarcophaga ruficornis lessThan /i greaterThan and lessThan i greaterThan Sarcophaga argyrostoma lessThan /i greaterThan were used to detect the visible light and fluorochrome interactions. Our results indicated that specific destruction of guanine residues is the main effect of photo-oxidation and LIB is a base-specific phenomenon. In addition, photo-oxidation may also cause DNA-protein cross-linking which affects the binding of some dyes, while prolonged photo-oxidation appears to cause more general damage to DNA. RASHMI SRIVASTAVA, PRATIMA GAUR1 AND NIDHI MISHRA Mitotic chromosomes, Sarcophaga species, Quinacrine, photo-oxidation 250-255