International Journal of Pharma and Bio Sciences
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10.22376/ijpbs.2019.10.1.p1-12
Volume 4 Issue 1
2013 (January - March)
A STUDY ON THE USE OF Α-AMYLASE INHIBITOR FROM VIGNA UNGUICULATA SEEDS AS AN ORGANIC NITROGEN SOURCE IN PLANT TISSUE CULTURE
α-Amylase inhibitor from lessThan i greaterThan Vigna unguiculata lessThan /i greaterThan seeds (VUAI) was purified using ion-exchange chromatography on Poros HS-50 column. Plant cell culture was used as it provides a controlled environmental and nutritional condition. VUAI used in different concentrations as 10, 100 and 200 ppm was found to increase the biomass of both grape and peanut callus cells in liquid media. Increased anthocyanin content in grape callus and total phenolics content in peanut callus was observed in VUAI treated groups. The VUAI treated groups showed better antioxidant activities than the control group. The SDS-PAGE profile showed a marked increase in the number of secreted proteins in the VUAI treated groups.
LAXMI H. GUPTA, D. RAJU, URMIL J. MEHTA AND SUSHMA G. SABHARWAL
Anthocyanin, Arachis hypogaea, biomass, polyphenols, Vitis vinifera
95-105