10.22376/ijpbs.2019.10.1.p1-12 Volume 1 Issue 1 2010 (January - March) lessThan i greaterThan Andrographis paniculata lessThan /i greaterThan (Family: Acanthaceae) is used- for its bitter tonic, stomachic, antipyretic and laxative properties in ayurveda. The present study consists of utilizing various enzymes and their combination to facilitate the release of andrographolide from polymeric cell matrix, which in turn will facilitate extraction of andrographolide from lessThan i greaterThan Andrographis lessThan /i greaterThan lessThan i greaterThan paniculata. lessThan /i greaterThan .Cellulase and Amylase enabled extraction of 0.37% and 0.34% of andrographolide. Cellulase was found to be most effective in releasing andrographolide as compared to other enzymes and enzyme blend. K.S.Laddha, Rajesh S.Gavit and Richie R.Bhandare Enzymes, Extraction, Andrographolide, Andrographis paniculata Nees. -