10.22376/ijpbs.2019.10.1.p1-12 Volume 3 Issue 3 2012 (July - September) Random Amplified Polymorphic DNA-Polymerase Chain Reaction (RAPD-PCR) technique has been used in the present study to investigate genetic relationship between the two muscids lessThan i greaterThan viz., lessThan /i greaterThan housefly lessThan i greaterThan Musca domestica lessThan /i greaterThan Linnaeus and lessThan i greaterThan Lispe orientalis lessThan /i greaterThan (Wiedemann). Tools for Population Genetic Analysis (TFPGA) software, was used to calculate genetic identity and distance, using data matrix prepared by the presence and absence of the bands generated by each primer. Average heterozygosity ranges from 0.156 to 0.301. The Nei's genetic identity was 0.322, which revealed rather low genetic identity between the two species. Malviya S, Bajpai, N. And Tewari, R.R. RAPD-PCR, Musca domestica, Lispe orientails, genetic relationship, Muscidae, heterozygosity. 1018-1024