10.22376/ijpbs.2019.10.1.p1-12 Volume 3 Issue 3 2012 (July - September) Mutations were induced in tissue cultured raised lessThan i greaterThan Bacopa monnieri lessThan /i greaterThan (L.) plantlets by treating lessThan i greaterThan in vitro lessThan /i greaterThan derived leaves with gamma rays at (γ – rays) dose rate of 0, 10, 20, 40 and 80 gray (Gy) and 0.5% of ethyl methanesulphonate (EMS) at 0, 0.5, 1.0, 1.5, 2.0 and 2.5 hours (h). Xantha mutants were observed with γ - rays at 10, 20 and 40 Gy. All the leaf explants treated with 80 Gy were either necrotic or irregenerable. Both γ and EMS treatment exhibited a reduction in survival rate, number of adventitious shoots, fresh weight and dry weight (DW). The γ - rays doses at 10, 20 and 40 Gy induced mutation in lessThan i greaterThan Bacopa monnieri lessThan /i greaterThan leaf explants and five lines were obtained with increased in the bacoside A content. The lines obtained are D15 (40 Gy) which produced maximum amount of bacoside A (22.567 mg g lessThan sup greaterThan -1 lessThan /sup greaterThan DW) followed by B11 (10 Gy), C18 (20 Gy), D17 (40 Gy) and B6 (10 Gy) lines which accumulated 21.051, 19.320, 17.163 and 14.133 mg g lessThan sup greaterThan -1 lessThan /sup greaterThan DW respectively compared with control (13.750 mg g lessThan sup greaterThan -1 lessThan /sup greaterThan DW). The leaf explants treated with EMS did not show any significant increase with respect to the bacoside A content. P. M. Naik, N. Praveen, S. H. Manohar And H. N. Murthy Bacopa monnieri, Bacoside A, Ethyl methanesulphonate, γ – rays, and High performance liquid chromatography. 848-855