10.22376/ijpbs.2019.10.1.p1-12 Volume 1 Issue 1 2010 (January - March) A simple sensitive isocratic method for the detection and quantification of mangostin in plasma has been developed. The assay consisted of reversed-phase HPLC with ultraviolet detection. Separation was achieved on a C lessThan sub greaterThan 18 lessThan /sub greaterThan reversed-phase column. The mobile phase consisting of methanol and water (95:5 %v/v) was delivered at a flow rate of 1.0 ml/min. The assay was shown to be linier over the range 4-100 µg/mL (r ≥ 0.9998). The HPLC analysis has been successfully applied to pharmacokinetic studies of mangostin after oral administration at a dose 40 mg/kgbwt to rats. The main pharmacokinetic parameters were: t lessThan sub greaterThan ½ lessThan /sub greaterThan 7.24 h ; K el 0.058/h ; t lessThan sub greaterThan max lessThan /sub greaterThan 62.99 min ; C lessThan sub greaterThan max lessThan /sub greaterThan 4.79 µg/mL; AUC 702.45 µg min/mL, respectively. SYAMSUDIN, FAIZATUN AND LESTARI RAHAYU Mangostin, HPLC, Pharmacokinetic studies and rat. -