10.22376/ijpbs.2019.10.1.p1-12 Volume 3 Issue 3 2012 (July - September) To investigate the effect of ethylacetate extract of lessThan i greaterThan  Aquilaria crassna lessThan /i greaterThan Pierre ex Lecomte on simulated ischemia-induced cardiac cell injury and its mechanism on p38 MAPK activation. Rat cardiac myoblast cell line (H9c2), were subjected to 2 hours simulated ischemia (sI) and 24 hours of reperfusion, in the presence and absence of the ethyl acetate extract of lessThan i greaterThan  Aquilaria crassna lessThan /i greaterThan (A.Q.). The cellular injury and viability were determined. The result found that A.Q. at concentration 5 mg/ml was the most effective concentration to reduce cell death and injury. Then, cells were pre-treated with or without 5 mg/ml of A.Q. for 1 hour before, or at the beginning of 40 minutes-sI. Activation of p38 MAPK was measured by Western blot analysis. Treatment of A.Q. significantly reduced ischemia-induced p38 MAPK phosphorylation ( lessThan i greaterThan p lessThan /i greaterThan lessThan 0.05). In conclusion, treatment of 5 mg/ml of the ethylacetate extract of lessThan i greaterThan  A. crassna lessThan /i greaterThan reduced ischemia induced cell death by attenuating p38 MAPK phosphorylation. Panadda Jermsri, Arunya Jiraviriyakul, Sasimanas Unajak And Sarawut Kumphune Aquilaria crassna; Ischemic heart disease; Myocardial Ischemia; cardiac cell; cell death; p38 MAPK 604-613