10.22376/ijpbs.2019.10.1.p1-12 Volume 3 Issue 3 2012 (July - September) Many bacteria produce extra cellular polysaccharide (EPS) and in genera such as lessThan i greaterThan Klebsiella, Rhizobium, Bacillus lessThan /i greaterThan and lessThan i greaterThan Streptococcus lessThan /i greaterThan they[Delete] have been well characterized for a number of species and strains. Polysaccharides from plant sources material and their modified forms are important additives in the food industry. The EPS is the use of xanthan in oil industry. The most significant biomedical exploitation of bacterial polysaccharides is used as vaccine agent. In the present study, the authors describe the selection of the plant lessThan i greaterThan Tephrosia purpurea lessThan /i greaterThan was selected[delete], since it contains many species producing EPS. From the root nodule of this plant, five different species of bacteria were isolated and studied for their antibacterial activity. Out of five strains, two strains showed promising activity against human pathogens ( lessThan i greaterThan Escherichia coli, Enterococcus aeruginosa, Klebsiella pneumoniae, Staphylococcus aureus lessThan /i greaterThan ) and these two strains were used for further studies. Molecular characterization for best strains was done and found to be lessThan i greaterThan Bacillus licheniformis lessThan /i greaterThan and lessThan i greaterThan Klebsiella pneumonia lessThan /i greaterThan . The growth curves of lessThan i greaterThan B.licheniformis lessThan /i greaterThan and lessThan i greaterThan K. pneumoniae lessThan /i greaterThan were studied. Also EPS were isolated and estimated by phenol sulphuric acid method in UV-Vis spectrophotometer. The large amounts of EPS 210 mg/ml from lessThan i greaterThan K.pneumoniae lessThan /i greaterThan and 70.02 mg/ml from lessThan i greaterThan B.licheniformis lessThan /i greaterThan were estimated before dialysis and after dialysis 12.3 mg/ml from lessThan i greaterThan K.pneumoniae lessThan /i greaterThan and 10.97 mg/ml from lessThan i greaterThan B.licheniformis lessThan /i greaterThan were estimated. Three polysaccharides components were observed by HPLC analysis. Also lyophilization was being used for the production of extra cellular polysaccharides. G. Balamurugan And S. Prakash EPS, Tephrosia purpurea, Bacillus licheniformis, Klebsiella pneumonia. 306-316