10.22376/ijpbs.2019.10.1.p1-12 Volume 3 Issue 3 2012 (July - September) L-Glutaminase [L-Glutaminase amido-hydrolase, EC-3.5.1.2] is widely distributed in microorganisms including bacteria, yeast, and fungi. The enzyme has received greater attention and significance in pharmaceutical industry since it is recognized as having potent antitumor activity. The enzyme also has applications in food industries. In the present work an extra cellular L-glutaminase was produced from lessThan i greaterThan Pseudomonas fluorescens lessThan /i greaterThan . The screening of extra cellular L-Glutaminase was done by dye based procedure on minimal agar medium. The conditions for production of glutaminase were optimized by studying the effect of incubation period and different carbon and nitrogen sources. The effect of temperature, pH and substrate concentration on L-glutaminase activity was studied. Maximum activity of enzyme was observed at 37 lessThan sup greaterThan 0 lessThan /sup greaterThan C and at pH 8. Chitanand M.PAnd Shete H.G. L-Glutaminase, L-Glutamine, Pseudomonas fluorescens 155-162