10.22376/ijpbs.2019.10.1.p1-12 Volume 3 Issue 3 2012 (July - September) A procedure for lessThan i greaterThan in vitro lessThan /i greaterThan culture of the economic important plant lessThan i greaterThan Aeschynomene indica lessThan /i greaterThan L. is described. A factorial experiment evaluated the effects of media Murashige and Skoog's medium (MS) and Gamberg lessThan i greaterThan et al lessThan /i greaterThan ., (B lessThan sub greaterThan 5 lessThan /sub greaterThan ) medium, temperatures (15° C and 20° C) presence or absence of light and plant growth regulators. Seed explants germinated in less than one week in culture and produced radicles. Optimal conditions for radical elongation were B lessThan sub greaterThan 5 lessThan /sub greaterThan at 20° C in the presence of light and without plant growth regulators. Plants were elongated on the same medium without plant growth regulators and acclimation to green house conditions. P.L.Indira1., S. Siva Subramanian, Junior Dyaku, M. Anitha, R.Karthikadevi, P. Pothammal, D. Suresh, A. Renuka, R.Balamani, V.Arulmathi And R.Kanimozhi Aeschynomene indica L., seed germination, radicle, Papilionaceae, in vitro 134-140