10.22376/ijpbs.2019.10.1.p1-12 Volume 3 Issue 3 2012 (July - September) The pathogenesis of Alzheimer's disease is complicated and related to COX-2. The NG108-15 cell line is a neuronal cell line. COX-2 expression in chemical induced neuronal differentiation of NG108-15 cells was investigated. Neuritogenesis of NG108-15 cells was induced by treatment with 100 mM dibutyryl cAMP (Bt lessThan sub greaterThan 2 lessThan /sub greaterThan cAMP) and 100 μM 12-o-tetradecanoyl-phorbol 13-acetate (TPA) for 3 days. Cellular morphology alteration was monitored microscopically; the level of COX-2 mRNA and protein expression was determined by RT-PCR and western blotting, respectively. The differentiated NG108-15 cells exhibited neuron-like morphology. Twenty four hours after induction, there was a transient up-regulation of both COX-2 mRNA and protein level. There was a slight increase in COX-2 mRNA level on day 2 of NG108-15 cells, without a significant different in COX-2 protein level. COX-2 mRNA and protein in NG108-15 cells were a transient up-regulation after differentiation by Bt lessThan sub greaterThan 2 lessThan /sub greaterThan cAMP and TPA. Prawpan Suwanakitch And Sukkid Yasothornsrikul Alzheimer’s disease, Cyclooxygenase 2 (COX-2), NG108-15 cells, Neurogenesis 74-81