10.22376/ijpbs.2019.10.1.p1-12 Volume 3 Issue 3 2012 (July - September) lessThan i greaterThan Luffa cylindrica lessThan /i greaterThan belongs to the family cucurbitaceae is an economically important plant widely grown for its vegetable purpose,but the plant as a whole is able to cure many of the skin diseases,thus possess medicinal importance also. In the present study callus multiplication of the luffa from the leaf explant have been investigated with different concentrations of auxin (NAA) with cytokinine (BAP) considering various parameters. The best result were obtained at BAP(1.5mg/L) and in combination with NAA at BAP(1.5mg/L) and NAA (1.0mg/L with maximum percent of callus which also initiated in short period of time of 10 to 12 days. Anu Shrivastava And Shikha Roy Callus, differentiation, Luffa cylindrica, cucurbitaceae. 526-531