10.22376/ijpbs.2019.10.1.p1-12 Volume 3 Issue 3 2012 (July - September) Using biological medium, such as plant extract for the biosynthesis of nanoparticles is an ecofriendly and emerging scientific trend. On this back drop the present study focused on the biosynthesis of silver nanoparticles using lessThan i greaterThan Cissus lessThan /i greaterThan lessThan i greaterThan quadrangularis lessThan /i greaterThan and evaluation of its antimicrobial and anticancer potentials. The biosynthesized nanoparticle was characterized by UV-VIS spectroscopy, EDAX and TEM. UV–visible spectrum of the aqueous medium containing silver nanoparticles showed a peak around 422 nm. The TEM and EDAX analysis showed that the particles were silver nanoparticles in the range of 5-30nm. The antimicrobial activity of the nanoparticles was studied against Gram positive and Gram negative bacteria. The cytotoxicity of the nanoparticles was studied against Hep2 and Vero cell line and the IC 50 value was found to be 64µg and 90 µg for Hep2 and Vero cell line respectively. K. Renugadevi, D. Inbakandan, M. Bavanilatha And V. Poornima Antimicrobial activity, anticancer activity, Biosynthesis, Silver nanoparticles, Cissus quadrangularis, Characterization. 437-445