10.22376/ijpbs.2019.10.1.p1-12 Volume 3 Issue 3 2012 (July - September) The purpose of this work was to evaluate the antimicrobial potential of endophytic fungi isolated from leaves of lessThan i greaterThan Camellia sinesis lessThan /i greaterThan plant. Many endophytes were isolated by using potato dextrose agar medium. In that one potent strain was taken for further study. The fungal culture was extracted with ethyl acetate and used as a crude extract for checking antimicrobial activities. The crude fungal extract showed different inhibitory activity against all pathogens. It showed significant activity against lessThan i greaterThan Pseudomonas lessThan /i greaterThan sp. The fungal strain was identified as lessThan i greaterThan Penicillium lessThan /i greaterThan sp. through 18S ribosome RNA sequence analysis. The lessThan i greaterThan Penicillium lessThan /i greaterThan sp. showed 100% gene sequence similarities with lessThan i greaterThan Penicillium funiculosumstrainTS08 and lessThan /i greaterThan lessThan i greaterThan Penicillium sp. lessThan /i greaterThan 12-2018S. The sequence of identified strain was submitted in NCBI and obtained accession number HQ214674 with name lessThan i greaterThan Penicillium lessThan /i greaterThan sp. pr NN-2010. The bioactive compounds responsible for antibacterial activity were identified by GC-MS and NMR. The major compound present in crude extract was identified as 3-(3-azidopropyl)-1H-indene (15.39%) and 3-Cyano-1,2-dimethylindole. In Nuclear magnetic resonance analysis most of the peaks range were between the aromatic groups. These results indicate that the bioactive metabolites isolated from fungal lessThan i greaterThan Penicillium lessThan /i greaterThan sp.could be promising source as antibacterial agents. Nameirakpam Nirjanta Devi And Femina Wahab Camellia sinesis, endophytic fungi, antimicrobial activity, GC-MS, NMR 420-427