International Journal of Pharma and Bio Sciences
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10.22376/ijpbs.2019.10.1.p1-12
Volume 3 Issue 3
2012 (July - September)
Estimation Of Granisetron Hydrochloride By A New Rp-Hplc Method
A simple, sensitive and specific reverse phase high performance liquid chromatographic method has been developed for the determination of granisetron hydrochloride in injectable dosage forms. Chromatographic separation was achieved on a peerless basic C18 (250×4.6 mm), 5.0 µm column with a diluted 1.6 ml of ortho phosphoric acid in 800 ml water, added 200ml acetonitrile, added 1.0ml of Hexylamine and mixed. Then adjusted the pH to 7.5 with triethylamine as mobile phase, detection was at 304 nm. Response was a linear function of concentration in the range 5-0.02 mg/L for granisetron hydrochloride. LOD and LOQ for granisetron hydrochloride were found 0.01 mg/L and 0.03 mg/L. Accuracy (recoveries 94-97%) and reproducibility were found to satisfactory.
T.Nageswara Rao, D.Sreenivasulu, P.U.V. Satish , T.Parvathamma,
Granisetron hydrochloride, RP-HPLC method, method validation
412-419